Aspects of Stage-by-Stage Diagnosis of Pulmonary Mycobacteriosis in Real Clinical Practice

The objective : to analyze the effectiveness of etiological verification pulmonary mycobacteriosis when using various biological materials at different diagnostic stages. Subjects and Methods . In Center for Diagnosis Rehabilitation Respiratory Diseases, CTRI, results stage-by-stage examinations 142 patients (from 2018 2022) were analyzed. A retrospective, prospective, cohort, open study was conducted. Inclusion criteria: etiologically verified by comprehensive microbiological (molecular genetic cultural tests) (with retrospective analysis after all stages examination). Exclusion HIV infection. 115 women 27 men enrolled in study, age ranged from 21 86 years, mean 57,9 years. All referred CTRI examination due newly detected changes lungs (bronchiectasis, focal changes, cavities). For diagnosis, sputum testing performed (Stage 1 examination); negative obtained, bronchoscopy prescribed bronchobiopsies used molecular diagnostics – Stage 2. case 2, patient recommended undergo a resection lung 3 examination. At stages, specimens sent testing: fluorescent microscopy detect acid-fact mycobacteria, real-time polymerase chain reaction (PCR-RV) NTBM DNA. Identification NTBMB carried out methods (PCR presence MTB DNA/NTBM (SYNTOL, Russia), on DNA strips (GenoType Mycobacterium CM\AS Hain Livescience, Germany)). Specimens cultured liquid medium ВАСТЕС MGIT960 (BD, USA). diagnosis established accordance with criteria proposed guidelines American Thoracic Society (ATS) Infectious Diseases America (IDSA) (2020): detection 2 samples or sample bronchobiopsy surgical [15]. studied data entered Microsoft Office Excel sheet (Microsoft, USA) statistical processing; level significance differences taken as 0.05. Results It found that slow-growing identified significant 93.0%, which 78.1% M. avium, fast-growing represented only abscessus, 7% observations. During X-ray examination, bronchiectatic form 52.1% (74/142) more often compared abdominal 30.9% (44/142) forms 16.9% (24/142) (p<0.001). When verifying test 91.0% (80/88). Regardless radiological disease dissemination disease, revealed pathology tracheobronchial tree 85.2% cases, mainly bronchitis purulent secretions.